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Fig. 8. BMP2 stimulates PAK1 and PAK4 activity. (A) C2C12 cells were stimulated with 3 nM BMP2 for different times and cell lysates were analyzed with the indicated phospho-specific antibodies, and membranes reprobed with anti-PAK1 and anti-PAK4 antibodies. Graphs indicate the relative PAK1-P and PAK4-P levels. Mean ± s.e.m. from three independent experiments (*P<0.05, paired t-test). (B) C2C12 cells were serum-starved, incubated with LY294002 and stimulated with 3 nM BMP2 for 40 minutes. Cell lysates were analyzed as above. Graph indicates the relative PAK1-P and PAK4-P levels. Mean ± s.e.m. from three independent experiments (*P<0.05, paired t-test). (C) C2C12 cells stimulated with 3 nM BMP2 and LY294002 were analyzed for PAK1 or PAK4 in vitro kinase activity using MBP as a substrate. The graph indicates the relative PAK1 and PAK4 activity. Mean ± s.e.m. from three independent experiments (*P<0.05, paired t-test).
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