spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 9


Fig. 9. Cdc42 is required for BMP2-dependent PAK1 and PAK4 phosphorylation. (A) C2C12 cells transfected with the indicated expression vectors were serum-starved for 16 hours and stimulated with 3 nM BMP2 for 40 minutes. Cell lysates were analyzed with the indicated phospho-specific antibodies, and membranes reprobed with anti-PAK1, anti-PAK4 and anti-Cdc42 antibodies. Graph indicates PAK1-P and PAK4-P levels of BMP2-treated cells relative to their respective untreated controls. Mean ± s.e.m. of three independent experiments (*P<0.05, unpaired t-test). (B) PAK activity is required for BMP2 to phosphorylate the LIMK1 associated to the BMPRII tail. Immunoblot analysis of C2C12 cells overexpressing His-tagged BMPRII under a tetracyclin (Tet-off)-regulated promoter (left panel). Cells overexpressing His-tagged BMPRII were transfected with the expression constructs indicated, serum-starved for 16 hours and stimulated with 3 nM BMP2 for 40 minutes. Cell extracts were subjected to immunoblotting to detect the total and phosphorylated LIMK1 after Ni2+-NTA-agarose purification (right panel).





Right arrow Return to article