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Figure 2


Fig. 2. Mto1-9A1 and Mto1-9A2 mutant proteins do not interact with the {gamma}-tubulin complex ({gamma}-TuC). (A) Anti-Myc immunoprecipitates of Myc-tagged wild-type and mutant Mto1 proteins (strains KS1517, KS1370, KS2290, KS2292, KS2294), probed with antibodies to the indicated proteins. The band migrating near Mto1-Myc on the anti-Myc blot of cell extract, `no tag' lane is an unrelated protein that is not precipitated by the anti-Myc antibody. IgG on the anti-{gamma}-tubulin blot is marked by asterisk. (B) Individual sections (S1, S2, S3) of a single interphase cell expressing Mto1-GFP and Alp4-tdTomato from endogenous promoters (strain KS3957). The SPB is apparent in S1. Note in S2 that some Mto1-GFP satellites do not contain visible Alp4-tdT (middle panel, arrowheads). (C-F) Maximum projections of cells expressing Mto1-GFP, Mto1-9A1-GFP, Mto1-9A2-GFP, or Mto1-9A3-GFP, together with Alp4-tdT (strains KS3957, KS3961, KS3962, KS3965) in interphase and mitosis. Bar, 10 µm.





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