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Figure 3


Fig. 3. AML1-ETO shows enhanced occupancy of rDNA repeats during mitosis. (A) Schematic of the RUNX consensus elements (ovals) in the human rDNA repeats depicting the locations of primers used for ChIP analysis. (B,C) ChIPs were done with antibodies for RUNX1/AML1, ETO and UBF1, as well as non-immune IgG in Kasumi-1 and Jurkat cells blocked in mitosis. An antibody detecting ETO was used to immunoprecipitate the AML1-ETO fusion protein, whereas an antibody recognizing the C-terminal domain of RUNX1/AML1 was used to pull down endogenous RUNX1/AML1. Quantitative PCR data are normalized to genomic DNA and denoted as percent input (note that y-axis scales vary).





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