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Figure 4


Fig. 4. Both RUNX1/AML1 and AML1-ETO occupy rDNA repeats in interphase Kasumi-1 cells. Chromatin immunoprecipitation was done in asynchronously growing HEL, Jurkat and Kasumi-1 cells using RUNX1/AML1, ETO, UBF1 and IgG antibodies. Three different PCR primer sets spanning RUNX consensus elements were used (see Fig. 3). (A,B) An antibody detecting ETO was used to immunoprecipitate the AML1-ETO fusion protein, whereas an antibody recognizing the C-terminal domain of RUNX1/AML1 was used to pull down endogenous RUNX1/AML1. Quantitative PCR data show that endogenous ETO does not bind to rDNA repeats in HEL cells nor in Jurkat cells (where ETO is not expressed), whereas UBF1 occupies the rDNA repeats in vivo in all the three cell lines tested. (C) RUNX1/AML1 and AML1-ETO both occupy rDNA repeats in Kasumi-1 cells. Quantitative PCR data are normalized to genomic DNA and denoted as percent input (note that y-axis scales vary).





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