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Figure 6


Fig. 6. RUNX1/AML1 associates with hypermethylated rDNA repeats. Chromatin from Kasumi cells was immunoprecipitated with antibodies against RUNX1/AML1 or AML1-ETO and the resulting DNA was digested with McrBC enzyme prior to qPCR using indicated rDNA primers. An antibody detecting ETO was used to immunoprecipitate the AML1-ETO fusion protein, whereas an antibody recognizing the C-terminal domain of RUNX1/AML1 was used to pull down endogenous RUNX1/AML1. Quantitative PCR data are normalized to genomic DNA and denoted as percentage input. These results indicate that rDNA repeats bound by RUNX1/AML1 are hypermethylated relative to those that are bound by AML1-ETO.





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