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Figure 7


Fig. 7. AML1-ETO presence on rDNA repeats correlates with altered histone H3 methylation. (A) AML1-ETO colocalizes with histone H3 dimethyl lysine 27 (H3K27) on mitotic chromosomes. Immunofluorescence microscopy with antibodies against post-translational modifications of nucleosomal histones in metaphase spreads prepared from Kasumi-1 cells. The antibodies used to detect histone modifications were as follows: H3K9me2, H3K4me2 and H3K27me2. Each group of five panels shows merged images co-stained with antibodies against AML1-ETO. The lower left panels in each group show DAPI staining only. The top three panels in each group (indicated by numbers) show enlargements of the areas numbered on the lower right of each group. (B) AML1-ETO occupancy of rDNA repeats is associated with histone H3 lysine 27 methylation (H3K27me2). ChIPs were performed with RUNX1/AML1, AML1-ETO, IgG and histone modification antibodies were done in Jurkat (left panels) and Kasumi-1 cells (right panels). The antibodies used to detect histone modifications were as follows: Acetylated histone H3 (Acet-H3), H3K4me2 and H3K27me2. Presence of AML1-ETO in Kasumi-1 cells correlates with elevated histone H3K27 methylation on the rDNA repeats compared with Jurkat cells, which express only RUNX1/AML1.





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