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Figure 8


Fig. 8. RUNX1/AML1 or AML1-ETO deficiency alters rRNA synthesis. Kasumi-1 cells were transfected with two independent RUNX1/AML1 or AML1-ETO siRNAs or non-silencing (NS) siRNA. (A) To check the efficiency of knockdown, protein expression of RUNX1/AML1, AML1-ETO and {alpha}-tubulin was examined by western blot analysis. (B) Expression of unprocessed rRNA (pre-rRNA synthesis), 28S RNA and p21 was examined by RT-PCR analysis. Bars represent expression levels relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA (± s.e.m.) from three independent experiments performed in triplicate. (C) Epitope-tagged RUNX1/AML1 and AML1-ETO were each ectopically expressed in mouse 32D myeloid progenitor cells. Pre-rRNA synthesis was measured by RT-qPCR relative to GAPDH in equal numbers of cells (top). Expression of the exogenous proteins was analyzed by western blot analysis (bottom). EV, empty vector.





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