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First published online November 19, 2008

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Mto1/2 gets microtubules going

During nucleation – an essential early step in de novo formation of microtubules – the -tubulin complex (-TuC) is recruited to prospective microtubule-organising centres. Relatively little is known about how this occurs, although it is thought that – in fission yeast – the interacting proteins Mto1 and Mto2 recruit -TuC. Ken Sawin and colleagues previously explored how knocking out mto1 or mto2 affected nucleation; now, on page 3971, they use site-directed mutagenesis of mto1 to further investigate the association of Mto1 and Mto2 with -TuC. The authors first show that, similar to mto1 mutants, cytoplasmic microtubule nucleation is abolished in fission yeast carrying a mutant mto1 with a disrupted centrosomin 1 motif (CM1) region; moreover, the mutant Mto1 does not interact with -TuC, although it localises normally and interacts with Mto2. By contrast, mutations outside the CM1 region of Mto1 phenocopy mto2 yeast – limited microtubule nucleation does occur, but binding of Mto1 to Mto2 or -TuC is inhibited. The authors next show that Mto1 and Mto2 form a -TuC-independent complex, and that each protein binds only weakly to -TuC in the absence of the other. They conclude that Mto1 and Mto2 act cooperatively to promote the association of the Mto1/2 complex with -TuC.

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Related articles in JCS:

Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the -tubulin complex in vivo

Itaru Samejima, Victoria J. Miller, Lynda M. Groocock, and Kenneth E. Sawin
JCS 2008 121: 3971-3980. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?