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First published online December 3, 2008
doi: 10.1242/10.1242/jcs.040667

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Melanosomes at a glance

¹ Molecular Medicine, National Heart and Lung Institute, Imperial College London, London SW7 2AZ, UK
² Instituto Gulbenkian de Ciência, 2780-156 Oeiras, Portugal
³ Faculdade de Ciências Médicas, Universidade Nova de Lisboa, 1169-056 Lisboa, Portugal

^* Author for correspondence (e-mail: m.seabra{at}imperial.ac.uk)

	Introduction

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
Melanosomes, the pigment granules that provide tissues with colour and photoprotection, are the cellular site of synthesis, storage and transport of melanin pigments. They are synthesised in mammalian skin melanocytes, in choroidal melanocytes and retinal pigment epithelial (RPE) cells in the eye, and in melanophores (a class of pigment-containing cells) in lower vertebrates. The precise fate and functions of melanosomes vary according to cell type – epidermal melanocytes supply neighbouring keratinocytes with melanosomes, which results in the pigmentation of skin and hair, whereas pigment granules are retained intracellularly in RPE cells and choroidal melanocytes. In lower vertebrates, the reversible aggregation and dispersion of melanosomes throughout the melanophore enables rapid colour change and adaptation to the environment.

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Box 1. The relationship between melanosomes and endosomes Melanosomes are lysosome-related organelles, which comprise a diverse group of specialised compartments, most of which contain secretory cargoes. Unlike classical secretory granules, lysosome-related organelles share some proteins (e.g. LAMP1) with lysosomes and appear to be derived from endosomes. The endosomal system is highly dynamic and consists of multiple functionally distinct compartments and subdomains. In melanocytes, some of these have been adapted to serve specialised roles in the biogenesis of melanosomes (Raposo and Marks, 2007). Nonpigmented stage I melanosomes are vacuolar early endosomes. They contain the melanosomal protein Pmel17, which is sorted into intralumenal vesicles (ILVs) within the organelle. A partial clathrin coat is seen on stage I melanosomes, and this might be involved in sorting proteins into ILVs of vacuolar endosomes (Clague, 2002), but it is not clear whether clathrin has a role in the trafficking of Pmel17 as this protein enters ILVs by a novel mechanism (Theos et al., 2006). Endosomal ILVs form in all cells; in melanocytes, however, the presence of Pmel17 gives rise to the structurally important intralumenal fibrils that characterise stage II melanosomes. The melanogenic enzymes tyrosinase and TYRP1 are delivered to stage II melanosomes. Thus, these proteins follow pathways that are distinct from those used by Pmel17. Again, the endosomal system is important here – tyrosinase and TYRP1 are now thought to traffic preferentially to melanosomes from early endosomes. They are present in tubular endosomal domains that are distinct from the regions occupied by Pmel17. Tyrosinase- and TYRP1-positive endosomal membranes have buds that are coated with the adaptor proteins AP1 or AP3, which is consistent with a role for these adaptors in sorting tyrosinase and TYRP1 to melanosomes (Theos et al., 2005). Recent studies have also implicated BLOC1 and BLOC2 in the regulation of endosome-to-melanosome transport (Di Pietro and Dell'Angelica, 2005; Setty et al., 2007). These widely expressed protein complexes are particularly important in the formation of lysosome-related organelles. Similar to AP1 and AP3, BLOC1 has been localised to tubular regions on early endosomes. Studies examining the trafficking of TYRP1 suggest that deficiencies in BLOC1 and AP3 have differential effects, which indicates that these complexes mediate distinct sorting pathways from the early endosome – despite some evidence for a physical interaction between them (Di Pietro and Dell'Angelica, 2005). By contrast, BLOC1 and BLOC2 appear to act in the same pathway. There are, however, subtle differences in their function and they might localise to partially distinct endosomal subdomains. Thus, BLOC1 might control the exit of cargo from early endosomes, with BLOC2 regulating a subsequent step – the direction of cargo to maturing melanosomes (Setty et al., 2007).

 

The major focus of this article is on mammalian skin pigmentation, including the regulation of pigment production, and the biogenesis of melanosomes and their intracellular and intercellular transport. Melanosomes in other well-studied systems, such as the mammalian retinal-pigment epithelium (RPE) and lower-vertebrate melanophores, are also briefly reviewed.

	Regulation of melanogenesis in skin melanocytes

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
The synthesis of melanin, in particular black eumelanin, is stimulated primarily by ultraviolet (UV) irradiation, which generates DNA photoproducts and leads to the release of various autocrine and paracrine factors, of which the most notable – -melanocyte-stimulating hormone (also known as proopiomelanocortin, gene symbol POMC and hereafter referred to as MSH) – is secreted by keratinocytes. MSH activates the melanocortin 1 receptor (MC1R) in the plasma membrane of skin melanocytes, which results in cAMP-dependent signalling and the stimulation of the expression of microphthalmia-associated transcription factor (MITF), a `master regulator' of melanocyte function and melanogenesis (Garcia-Borron et al., 2005; Vance and Goding, 2004). The activity of MITF contributes to the expression of a host of genes that are involved in melanocyte survival [cyclin-dependent kinase 2 (Cdk2), p16INK4a, T-box transcription factor 2 (Tbx2) and p21 (CDKN1A)], motility (Met), differentiation and apoptosis [Bcl2 and hypoxia-inducible factor 1a (Hif1a)], and in melanosome production [tyrosinase, tyrosinase-like protein 1 (Tyrp1), dopachrome tautomerase (Dct), melanoma antigen recognised by T cells melan-A (Mlana; also known as MART1)], absent in melanoma 1 (Aim1) and melanocyte protein Pmel17 (Silv) and transport (Rab27a) (Chiaverini et al., 2008; Levy et al., 2006).

	Melanosome biogenesis

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
Classical electron microscopy studies of skin melanocytes have distinguished four morphologically distinct stages of melanosome development, and a similar developmental progression appears to occur in other pigmented cell types such as RPE cells (Lopes et al., 2007b). Stage I pre-melanosomes are nonpigmented vacuoles that are derived from the endosomal system. These then acquire characteristic internal striations (stage II). Melanin pigment is deposited onto the striations (stage III), eventually giving rise to mature, fully melanised stage IV melanosomes (Raposo and Marks, 2007). Progress in unravelling the complex trafficking pathways of melanosomal proteins that underlie this maturation process is providing new insights into how melanosomes are formed. For example, Pmel17, an important structural component of melanosomes, is present in the limiting membrane of stage I pre-melanosomes then enters intralumenal vesicles, undergoes proteolytic cleavage and forms the lumenal fibrillar striations that characterise stage II organelles (Harper et al., 2008; Raposo and Marks, 2007; Theos et al., 2006; Valencia et al., 2007). Further examples are the key melanogenic enzymes tyrosinase and TYRP1: these transmembrane proteins travel via the secretory pathway and early endosomal intermediates (Hearing, 2005; Raposo and Marks, 2007). From there, they are delivered to preformed stage II melanosomes, where they initiate pigment synthesis and further maturation.

Mouse coat-colour mutants have proved invaluable for the identification of proteins that regulate melanosome biogenesis and maturation (Bennett and Lamoreux, 2003) (see also www.espcr.org/micemut for a recent summary). Most coat-colour-pigmentation mutants in this category represent models for Hermansky-Pudlak syndrome (HPS), a phenotype that can be caused by mutations in a number of genes that are implicated in the biogenesis of lysosome-related organelles in several tissues (Di Pietro and Dell'Angelica, 2005; Gautam et al., 2006; Wei, 2006). Two such mouse models (pearl and mocha) exhibit mutations in the gene encoding the adaptor complex protein AP3. Analysis of AP3-mutant cells revealed its involvement in the transport of tyrosinase from early endosomes to melanosomes (Huizing et al., 2001; Theos et al., 2005). Interestingly, an AP1-dependent alternative route also exists, and this partially compensates for a loss of AP3, which illustrates the flexibility and functional redundancy of protein trafficking in melanocytes (Huizing et al., 2001; Theos et al., 2005).

Other models for HPS carry mutations in the genes encoding biogenesis of lysosome-related organelles complexes (BLOCs) 1, 2 and 3 (Dell'Angelica, 2004), or in the homotypic fusion and vacuole protein sorting (HOPS) complex (also known as the class C Vps complex), which is involved in fusion of endosomal organelles in mammalian cells (Richardson et al., 2004). BLOC1 and BLOC2 are two distinct protein complexes that are required for the sorting of melanogenic enzymes from endosomes to melanosomes. In the absence of BLOC1 or BLOC2, TYRP1 is mislocalised and accumulates in early endosomes (Di Pietro et al., 2006; Setty et al., 2007). The roles of BLOC3 and the HOPS complex in melanosomal protein trafficking are poorly understood. The closely related tissue-specific small GTPases Rab38 and Rab32 also have crucial roles in pigmentation; they localise to cytoplasmic vesicles and mature melanosomes, and regulate the post-Golgi transport of tyrosinase and TYRP1 to melanosomes (Loftus et al., 2002; Wasmeier et al., 2006). Additional proteins that are implicated in melanosome biogenesis include MART1 (De Mazière et al., 2002; Hoashi et al., 2005), ocular albinism type 1 protein (OA1; also known as G-protein-coupled receptor 143) (Palmisano et al., 2008; Samaraweera et al., 2001; Schiaffino and Tacchetti, 2005), oculocutaneous albinism II (OCA2; also known as P protein) (Brilliant, 2001) and AIM1 (Costin et al., 2003); these might localise to endosomes and/or early melanosomes, but their precise roles remain to be established.

	Melanosome transport in skin melanocytes

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
Histological examination of mammalian skin reveals that melanocytes reside adjacent to the epidermal basement membrane and extend multiple dendritic extensions that act as conduits, allowing the transport of mature melanosomes from their site of synthesis in the melanocyte perinuclear region to numerous surrounding keratinocytes (Nascimento et al., 2003; Seabra and Coudrier, 2004; Van Den Bossche et al., 2006). The molecular mechanisms of intracellular melanosome transport between the perinuclear region and dendrite tips have served as paradigms for organelle motility in general. Video-microscopy studies of melanosome movements in myosin VA (Myo5a)-null melanocytes led to the proposal of a two-step transport system for mature melanosomes, in which rapid microtubule (MT)-dependent long-distance transport to the tip of the dendrite is followed by MYO5A- and actin-dependent `capture' at the tip (Wu et al., 1998). A combination of genetic and cell-biological studies has shown that the small GTPase Rab27a and its effector melanophilin (Mlph; also known as Slac2a and exophilin 3) act as an organelle-associated receptor for a splice variant of MYO5A that is expressed in melanocytes (Hammer and Wu, 2002; Seabra and Coudrier, 2004). The Rab27a-Mlph-MYO5A complex promotes the association of melanosomes with cortical actin within the dendrites of melanocytes, promoting their detachment from MTs and positioning them close the plasma membrane. A recent study also suggested that OA1 functions to regulate actin-dependent melanosome distribution, in addition to its role in melanosome biogenesis (Palmisano et al., 2008).

	Intercellular melanosome transfer in the skin

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
Following their biogenesis and transport, melanosomes pass from the dendrites of melanocytes to neighbouring keratinocytes in a process that remains poorly understood, despite numerous attempts to study melanosome transfer by using in vitro co-culture systems (Berens et al., 2005; Minwalla et al., 2001; Virador et al., 2002). There are several current hypotheses about how intercellular transfer occurs – possible mechanisms include the cytophagocytosis of dendrite tips by keratinocytes, the exocytosis of melanin cores from melanocytes followed by their phagocytic uptake into keratinocytes, the transfer of melanosomes between the two cell types via membrane-bound vesicles, and the direct fusion of melanocyte and keratinocyte plasma membranes (Van Den Bossche et al., 2006). Melanosome transfer might be stimulated by physical contact between melanocytes and keratinocytes; this might activate a paracrine signalling loop and/or the production of transient intracellular calcium signals within keratinocytes (Joshi et al., 2007; Virador et al., 2002). Keratinocyte cell-surface receptors, including the proteinase-activated receptor 2 (PAR2) and the keratinocyte growth factor receptor (KGFR; also known as fibroblast growth factor receptor 2), have also been identified as possible regulators of melanosome internalisation (Boissy, 2003; Cardinali et al., 2005). It is noteworthy that melanocyte dendrites morphologically resemble the recently discovered tunnelling nanotubes, which form between cells of several types in culture (such as PC12 cells, dendritic cells and T cells) and might be involved in intercellular transfer of organelles, plasma membrane and cytoplasmic molecules (Gerdes and Carvalho, 2008). Thus, the elucidation of the mechanism of melanosome transfer between melanocytes and keratinocytes might provide wide-ranging insights into the process of intercellular transport.

	Melanosomes in the RPE

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
RPE cells in the eye form a polarised epithelium between photoreceptor cells and the underlying choroid, and possess actin-rich apical processes that interdigitate between the outer segments of the photoreceptors. They phagocytose the tips of photoreceptor outer segments, are required for the regeneration of the visual pigment retinal and help to maintain the blood-retina barrier (Futter, 2006; Strauss, 2005). Unlike melanosome synthesis in skin melanocytes, which occurs continuously, melanosome biogenesis in mouse RPE cells is completed before birth and pigment granules are retained throughout life (Lopes et al., 2007b). EM studies reveal that there is a net transport of melanosomes from the cell body into apical processes in response to light onset (Futter et al., 2004). This might be governed by the assembly of a complex comprising Rab27a, myosin- and Rab-interacting protein (Myrip) and MYO5A. This complex is similar to the Rab27a-Mlph-MYO5A complex that is required for the retention of melanosomes in the dendrites of skin melanocytes (see above), and appears to perform a similar function in allowing MT-associated melanosomes to be transferred onto actin filaments and to move into apical processes (Futter, 2006; Gibbs et al., 2004; Lopes et al., 2007a).

	Melanosome transport in melanophores

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
The major difference between melanosome transport in mammals and lower vertebrates (such as frog and fish) is that the melanophores of poikilothermic vertebrates can acutely and reversibly regulate pigment-granule movement in response to extracellular stimuli. Melatonin or MSH that are produced by the pituitary gland mediate changes in cytosolic cyclic adenosine 3',5'-monophosphate (cAMP) levels within melanophores that result in rapid (within 15-60 minutes) perinuclear aggregation or peripheral dispersion of melanosomes, respectively (Nascimento et al., 2003). As in mammalian cells, melanosomes are proposed to move by means of a coupled system of MT-dependent transport to the periphery, followed by actin-dependent retention. Biochemical studies of melanosomes purified from aggregated and dispersed states indicate that motor proteins remain attached to the melanosome even when their activity is not required; for example, kinesin II remains associated with melanosomes in an inactive state during melanocortin-induced pigment aggregation (Reilein et al., 1998). In contrast to mammalian cells, however, motor activity in melanophores is acutely altered in a cAMP- and protein kinase A (PKA)-dependent manner. Elevated cAMP results in increased kinesin and MYO5A activity, which leads to melanosome dispersion, whereas reduced cAMP results in increased dynein activity, which gives rise to aggregation (Rodionov et al., 2003). PKA directly associates with melanosomal kinesin and dynein and might be recruited to melanosomes by the small GTPase Rab32 (Kashina et al., 2004; Park et al., 2007). Recent studies of melanophilin (Mlpha)-knockout melanophores in zebrafish suggest that Mlpha promotes PKA-mediated melanosome dispersion by regulating the activity of both MYO5A and dynein (Sheets et al., 2007).

	Conclusions

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 
Melanosomes are intriguing organelles that are involved in diverse cellular processes, and their study has provided new insights into a variety of cell-biological and physiological issues. As model lysosome-related organelles, melanosomes can contribute to a better understanding of related organelles and of the highly specialised cell types that harbour them. The identification and characterisation of melanosomal proteins and regulators of melanosome function through genetic, functional and proteomic studies (Chi et al., 2006), therefore, continues to be a topic of great interest.

	References

Top Introduction Regulation of melanogenesis in... Melanosome biogenesis Melanosome transport in skin... Intercellular melanosome... Melanosomes in the RPE Melanosome transport in... Conclusions References

 

Bennett, D. C. and Lamoreux, M. L. (2003). The color loci of mice-a genetic century. Pigment Cell Res. 16, 333-344.[CrossRef][Medline]

Berens, W., Van Den Bossche, K., Yoon, T. J., Westbroek, W., Valencia, J. C., Out, C. J. J. M. N., Hearing, V. J. and Lambert, J. (2005). Different approaches for assaying melanosome transfer. Pigment Cell Res. 18, 370-381.[CrossRef][Medline]

Boissy, R. E. (2003). Melanosome transfer to and translocation in the keratinocyte. Exp. Dermatol. 12, 5-12.[CrossRef]

Brilliant, M. H. (2001). The mouse p (pink-eyed dilution) and human P genes, oculocutaneous albinism type 2 (OCA2), and melanosomal pH. Pigment Cell Res. 14, 86-93.[CrossRef][Medline]

Cardinali, G., Ceccarelli, S., Kovacs, D., Aspite, N., Lotti, L. V., Torrisi, M. R. and Picardo, M. (2005). Keratinocyte growth factor promotes melanosome transfer to keratinocytes. J. Invest. Dermatol. 125, 1190-1199.[CrossRef][Medline]

Chi, A., Valencia, J. C., Hu, Z. Z., Watabe, H., Yamaguchi, H., Mangini, N. J., Huang, H., Canfield, V. A., Cheng, K. C., Yang, F. et al. (2006). Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. J. Proteome Res. 5, 3135-3144.[CrossRef][Medline]

Chiaverini, C., Beuret, L., Flori, E., Busca, R., Abbe, P., Bille, K., Bahadoran, P., Ortonne, J. P., Bertolotto, C. and Ballotti, R. (2008). Microphthalmia-associated transcription factor regulates RAB27A gene expression and controls melanosome transport. J. Biol. Chem. 283, 12635-12642.[Abstract/Free Full Text]

Costin, G. E., Valencia, J. C., Vieira, W. D., Lamoreux, M. L. and Hearing, V. J. (2003). Tyrosinase processing and intracellular trafficking is disrupted in mouse primary melanocytes carrying the underwhite (uw) mutation: a model for oculocutaneous albinism (OCA) type 4. J. Cell Sci. 116, 3203-3212.[Abstract/Free Full Text]

De Mazière, A. M., Muehlethaler, K., van Donselaar, E., Salvi, S., Davoust, J., Cerottini, J. C., Lévy, F., Slot, J. W. and Rimoldi, D. (2002). The melanocytic protein Melan-A/MART-1 has a subcellular localization distinct from typical melanosomal proteins. Traffic 3, 678-693.[CrossRef][Medline]

Dell'Angelica, E. C. (2004). The building BLOC(k)s of lysosomes and related organelles. Curr. Opin. Cell Biol. 16, 458-464.[CrossRef][Medline]

Di Pietro, S. M. and Dell'Angelica, E. C. (2005). The cell biology of Hermansky, Pudlak syndrome: recent advances. Traffic 6, 525-533.[CrossRef][Medline]

Di Pietro, S. M., Falcón-Pérez, J. M., Tenza, D., Setty, S. R., Marks, M. S., Raposo, G. and Dell'Angelica, E. C. (2006). BLOC-1 interacts with BLOC-2 and the AP-3 complex to facilitate protein trafficking on endosomes. Mol. Biol. Cell 17, 4027-4038.[Abstract/Free Full Text]

Futter, C. E. (2006). The molecular regulation of organelle transport in mammalian retinal pigment epithelial cells. Pigment Cell Res. 19, 104-111.[CrossRef][Medline]

Futter, C. E., Ramalho, J. S., Jaissle, G. B., Seeliger, M. W. and Seabra, M. C. (2004). The role of Rab27a in the regulation of melanosome distribution within retinal pigment epithelial cells. Mol. Biol. Cell 15, 2264-2275.[Abstract/Free Full Text]

Garcia-Borron, J. C., Sanchez-Laorden, B. L. and Jimenez-Cervantes, C. (2005). Melanocortin-1 receptor structure and functional regulation. Pigment Cell Res. 18, 393-410.[Medline]

Gautam, R., Novak, E. K., Tan, J., Wakamatsu, K., Ito, S. and Swank, R. T. (2006). Interaction of Hermansky-Pudlak Syndrome genes in the regulation of lysosome-related organelles. Traffic 7, 779-792.[CrossRef][Medline]

Gerdes, H. H. and Carvalho, R. N. (2008). Intercellular transfer mediated by tunneling nanotubes. Curr. Opin. Cell Biol. 20, 470-475.[CrossRef][Medline]

Gibbs, D., Azarian, S. M., Lillo, C., Kitamoto, J., Klomp, A. E., Steel, K. P., Libby, R. T. and Williams, D. S. (2004). Role of myosin VIIa and Rab27a in the motility and localization of RPE melanosomes. J. Cell Sci. 117, 6473-6483.[Abstract/Free Full Text]

Hammer, J. A., 3rd and Wu, X. S. (2002). Rabs grab motors: defining the connections between Rab GTPases and motor proteins. Curr. Opin. Cell Biol. 14, 69-75.[CrossRef][Medline]

Harper, D. C., Theos, A. C., Herman, K. E., Tenza, D., Raposo, G. and Marks, M. S. (2008). Premelanosome amyloid-like fibrils are composed of only golgi-processed forms of Pmel17 that have been proteolytically processed in endosomes. J. Biol. Chem. 283, 2307-2322.[Abstract/Free Full Text]

Hearing, V. J. (2005). Biogenesis of pigment granules: a sensitive way to regulate melanocyte function. J. Dermatol. Sci. 37, 3-14.[CrossRef][Medline]

Hoashi, T., Watabe, H., Muller, J., Yamaguchi, Y., Vieira, W. D. and Hearing, V. J. (2005). MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes. J. Biol. Chem. 280, 14006-14016.[Abstract/Free Full Text]

Huizing, M., Sarangarajan, R., Strovel, E., Zhao, Y., Gahl, W. A. and Boissy, R. E. (2001). AP-3 mediates tyrosinase but not TRP-1 trafficking in human melanocytes. Mol. Biol. Cell 12, 2075-2085.[Abstract/Free Full Text]

Joshi, P. G., Nair, N., Begum, G., Joshi, N. B., Sinkar, V. P. and Vora, S. (2007). Melanocyte-keratinocyte interaction induces calcium signalling and melanin transfer to keratinocytes. Pigment Cell Res. 20, 380-384.[Medline]

Kashina, A. S., Semenova, I. V., Ivanov, P. A., Potekhina, E. S., Zaliapin, I. and Rodionov, V. I. (2004). Protein kinase A, which regulates intracellular transport, forms complexes with molecular motors on organelles. Curr. Biol. 14, 1877-1881.[CrossRef][Medline]

Levy, C., Khaled, M. and Fisher, D. E. (2006). MITF: master regulator of melanocyte development and melanoma oncogene. Trends Mol. Med. 12, 406-414.[CrossRef][Medline]

Loftus, S. K., Larson, D. M., Baxter, L. L., Antonellis, A., Chen, Y., Wu, X., Jiang, Y., Bittner, M., Hammer, J. A., 3rd and Pavan, W. J. (2002). Mutation of melanosome protein RAB38 in chocolate mice. Proc. Natl. Acad. Sci. USA 99, 4471-4476.[Abstract/Free Full Text]

Lopes, V. S., Ramalho, J. S., Owen, D. M., Karl, M. O., Strauss, O., Futter, C. E. and Seabra, M. C. (2007a). The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epithelium. Traffic 8, 486-499.[CrossRef][Medline]

Lopes, V. S., Wasmeier, C., Seabra, M. C. and Futter, C. E. (2007b). Melanosome maturation defect in Rab38-deficient retinal pigment epithelium results in instability of immature melanosomes during transient melanogenesis. Mol. Biol. Cell 18, 3914-3927.[Abstract/Free Full Text]

Minwalla, L., Zhao, Y., Cornelius, J., Babcock, G. F., Wickett, R. R., Le Poole, I. C. and Boissy, R. E. (2001). Inhibition of melanosome transfer from melanocytes to keratinocytes by lectins and neoglycoproteins in an in vitro model system. Pigment Cell Res. 14, 185-194.[CrossRef][Medline]

Nascimento, A. A., Roland, J. T. and Gelfand, V. I. (2003). Pigment cells: a model for the study of organelle transport. Annu. Rev. Cell Dev. Biol. 19, 469-491.[CrossRef][Medline]

Palmisano, I., Bagnato, P., Palmigiano, A., Innamorati, G., Rotondo, G., Altimare, D., Venturi, C., Sviderskaya, E. V., Piccirillo, R., Coppola, M. et al. (2008). The ocular albinism type 1 protein, an intracellular G protein-coupled receptor, regulates melanosome transport in pigment cells. Hum. Mol. Genet. 17, 3487-3501.[Abstract/Free Full Text]

Park, M., Serpinskaya, A. S., Papalopulu, N. and Gelfand, V. I. (2007). Rab32 regulates melanosome transport in Xenopus melanophores by protein kinase a recruitment. Curr. Biol. 17, 2030-2034.[CrossRef][Medline]

Raposo, G. and Marks, M. S. (2007). Melanosomes-dark organelles enlighten endosomal membrane transport. Nat. Rev. Mol. Cell. Biol. 8, 786-797.[CrossRef][Medline]

Reilein, A. R., Tint, I. S., Peunova, N. I., Enikolopov, G. N. and Gelfand, V. I. (1998). Regulation of organelle movement in melanophores by protein kinase A (PKA), protein kinase C (PKC), and protein phosphatase 2A (PP2A). J. Cell Biol. 142, 803-813.[Abstract/Free Full Text]

Richardson, S. C., Winistorfer, S. C., Poupon, V., Luzio, J. P. and Piper, R. C. (2004). Mammalian late vacuole protein sorting orthologues participate in early endosomal fusion and interact with the cytoskeleton. Mol. Biol. Cell 15, 1197-1210.[Abstract/Free Full Text]

Rodionov, V., Yi, J., Kashina, A., Oladipo, A. and Gross, S. P. (2003). Switching between microtubule- and actin-based transport systems in melanophores is controlled by cAMP levels. Curr. Biol. 13, 1837-1847.[CrossRef][Medline]

Samaraweera, P., Shen, B., Newton, J. M., Barsh, G. S. and Orlow, S. J. (2001). The mouse ocular albinism 1 gene product is an endolysosomal protein. Exp. Eye Res. 72, 319-329.[CrossRef][Medline]

Schiaffino, M. V. and Tacchetti, C. (2005). The ocular albinism type 1 (OA1) protein and the evidence for an intracellular signal transduction system involved in melanosome biogenesis. Pigment Cell Res. 18, 227-233.[CrossRef][Medline]

Seabra, M. C. and Coudrier, E. (2004). Rab GTPases and myosin motors in organelle motility. Traffic 5, 393-399.[CrossRef][Medline]

Setty, S. R., Tenza, D., Truschel, S. T., Chou, E., Sviderskaya, E. V., Theos, A. C., Lamoreux, M. L., Di Pietro, S. M., Starcevic, M., Bennett, D. C. et al. (2007). BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles. Mol. Biol. Cell 18, 768-780.[Abstract/Free Full Text]

Sheets, L., Ransom, D. G., Mellgren, E. M., Johnson, S. L. and Schnapp, B. J. (2007). Zebrafish melanophilin facilitates melanosome dispersion by regulating dynein. Curr. Biol. 17, 1721-1734.[CrossRef][Medline]

Strauss, O. (2005). The retinal pigment epithelium in visual function. Physiol. Rev. 85, 845-881.[Abstract/Free Full Text]

Theos, A. C., Tenza, D., Martina, J. A., Hurbain, I., Peden, A. A., Sviderskaya, E. V., Stewart, A., Robinson, M. S., Bennett, D. C., Cutler, D. F. et al. (2005). Functions of adaptor protein (AP)-3 and AP-1 in tyrosinase sorting from endosomes to melanosomes. Mol. Biol. Cell 16, 5356-5372.[Abstract/Free Full Text]

Theos, A. C., Truschel, S. T., Tenza, D., Hurbain, I., Harper, D. C., Berson, J. F., Thomas, P. C., Raposo, G. and Marks, M. S. (2006). A lumenal domain-dependent pathway for sorting to intralumenal vesicles of multivesicular endosomes involved in organelle morphogenesis. Dev. Cell 10, 343-354.[CrossRef][Medline]

Valencia, J. C., Rouzaud, F., Julien, S., Chen, K. G., Passeron, T., Yamaguchi, Y., Abu-Asab, M., Tsokos, M., Costin, G. E., Yamaguchi, H. et al. (2007). Sialylated core 1 O-glycans influence the sorting of Pmel17/gp100 and determine its capacity to form fibrils. J. Biol. Chem. 282, 11266-11280.[Abstract/Free Full Text]

Van Den Bossche, K., Naeyaert, J. M. and Lambert, J. (2006). The quest for the mechanism of melanin transfer. Traffic 7, 769-778.[CrossRef][Medline]

Vance, K. W. and Goding, C. R. (2004). The transcription network regulating melanocyte development and melanoma. Pigment Cell Res. 17, 318-325.[CrossRef][Medline]

Virador, V. M., Muller, J., Wu, X., Abdel-Malek, Z. A., Yu, Z. X., Ferrans, V. J., Kobayash, i. N., Wakamatsu, K., Ito, S., Hammer, J. A. et al. (2002). Influence of alpha-melanocyte-stimulating hormone and ultraviolet radiation on the transfer of melanosomes to keratinocytes.. FASEB J. 16, 105-107.[Abstract/Free Full Text]

Wasmeier, C., Romao, M., Plowright, L., Bennett, D. C., Raposo, G. and Seabra, M. C. (2006). Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes. J. Cell Biol. 175, 271-281.[Abstract/Free Full Text]

Wei, M. L. (2006). Hermansky-Pudlak syndrome: a disease of protein trafficking and organelle function. Pigment Cell Res. 19, 19-42.[CrossRef][Medline]

Wu, X., Bowers, B., Rao, K., Wei, Q. and Hammer, J. A., 3rd. (1998). Visualization of melanosome dynamics within wild-type and dilute melanocytes suggests a paradigm for myosin V function in vivo. J. Cell Biol. 143, 1899-1918.[Abstract/Free Full Text]

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