spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. GFP-fusion proteins and their treatment with PI-PLC. (A) In the fusion constructs GFP-FR and GFP-PrP, GFP is fused to the GPI-anchor attachment signal of folate receptor (FR) and prion protein (PrP), respectively. The FR or PrP sequence included in the fusion proteins is shown. The {omega}-site is shown in red. (B) MDCK cells stably expressing GFP-FR or GFP-PrP were extracted with Triton X-114. After phase separation, detergent phases were incubated in the presence (+) or absence (–) of PI-PLC (15 µg/ml) as previously described (Lisanti et al., 1989). The resulting aqueous phases were precipitated with trichloroacetic acid and detected by western blotting using anti-GFP antibody. As previously shown, the band migrating at 43 kDa represents a partially denatured dimer of GFP (Paladino et al., 2004).





Right arrow Return to article