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Figure 2


Fig. 2. Clathrin depletion does not appear to block internalization of Arf6 pathway cargoes, but alters their intracellular distribution. (A) Live clathrin-depleted cells were incubated with an antibody against the extracellular domain of MHCI for 4 hours, then fixed and subjected to immunofluorescence using anti-CHC antibody. (B) Clathrin-depleted cells were incubated with antibody against the extracellular domain of β1 integrin for 3.5 hours. Alexa-Fluor-546–Tfn was then added and cells were incubated for an additional 30 minutes. Samples were fixed and analyzed by confocal microscopy. Arrows indicate depleted cells.





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