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Figure 8


Fig. 8. AP-2 depletion targets Arf6 cargo to CD63- and Lamp1-positive endosomes. (A) Cells depleted of {alpha}-adaptin were subjected to indirect immunofluorescence using antibodies against (A) CD63 and Lamp1, (B) EEA1, (C) TfnR or (D) TfnR and CD63. In B and C, live cells were incubated with antibody against β1 integrin for 4 hours prior to fixation. Arrows in A and D indicate depleted cells; cells shown in B and C are depleted of {alpha}-adaptin.





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