|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. Comparison of telomere and centromere spatial organization in 35y and 81y hMSCs. The 35y and 81y cells were isolated from two individuals at age 35 (35y) and 81 (81y). (A) Formation of telomere aggregates in 81y cells at passage 6. Cells at passage 5 were transduced with TRF1-DsRed lentiviral vectors. 3D B-box plots show the ratio in the fluorescent intensity between small telomere aggregates and normal telomeres [(t<i<T)/(i<t)], the ratio in fluorescent intensity between large telomere aggregates and normal telomeres [(i>T)/(i<t)] and the ratio between the number of dots found in aggregates to total TRF1 fluorescent dots. CDF plots (bottom left) show the normalized frequency as a function of a normalized nucleus radius. The P value (KS Test) is indicated and suggests that the underlying distributions differ significantly. Statistical analyses represent 1750 TRF1-DsRed fluorescent dots obtained from 25 cells per cell class. (B) CDF plots of CenpA spatial localization in 35y and 81y cells. Cells at passage 5 were transduced with CenpA-EGFP lentivirus vectors. Confocal images were taken from living cells at passage 6. The plots show the normalized frequency as a function of a normalized nucleus radius. The P value (KS Test) is indicated and suggests that the underlying distributions differ significantly. Statistical analyses represent 525 CenpA-fluorescent dots obtained from 15 cells per cell class.
|
