spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. AQP4 knockout downregulates the expression of Cav1.2 and connexin43 in ANSCs. (A) RT-PCR analysis shows Cav1.2, Cav3.1 and TRPC1 mRNA is present in the ANSCs. The transcripts of Cav1.3, Cav3.2 and Cav3.3 were not detectable. (B) In Aqp4–/– ANSCs, the mRNAs of genes encoding Cav1.2 and Cav3.1 were significantly decreased when normalized to GAPDH (*P<0.05, n=3). There was no significant inhibition in the transcription of TRPC1 in Aqp4–/– ANSCs compared with the Aqp4+/+ control. (C) Western blot confirms that AQP4 knockout decreased the expression of Cav1.2 channel protein in the ANSCs compared with the wild type (*P<0.05, n=3). Cav3.1 channel protein was not detected. (D) Western blotting (top panel) showing that the expression level of Cx43 protein in Aqp4–/– ANSCs decreased to 39% of the level in the Aqp4+/+ genotype (lower panel) (*P<0.01, n=3).





Right arrow Return to article