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Figure 1


Fig. 1. UVC-induced phosphorylation of eIF2{alpha}. Immunoblots of total cell extracts probed with antibody against eIF2{alpha}-P and against Cdc2 as loading control, and the quantification (bottom). (A) G1-synchronised cdc10-M17 cells exposed to increasing doses of UVC irradiation. (B) Asynchronous cells auxotrophic for leucine, which were starved of leucine for 2 hours (–Leu) and asynchronous wild-type cells exposed to 0 or 1100 J/m2 of UVC. (C) Synchronous cdc10-M17 cells in G1, S and G2 phase exposed to 0 and 1100 J/m2 of UVC. The corresponding DNA histograms are shown below. (D) G1-synchronised cdc10-M17 cells irradiated with 0 (control) or 1100 J/m2 of UVC and incubated for different times after irradiation, as indicated.





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