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Figure 2


Fig. 2. H2O2-induced eIF2{alpha} phosphorylation and cell cycle delay. (A) Immunoblot (see Fig. 1 for details) of asynchronous cells exposed to increasing concentrations of H2O2. G1-synchronised wild-type (B) and gcn2 mutant (C) cells exposed to 0 (control) or 5 mM H2O2 and incubated for different times, as indicated. The quantified relative value for H2O2 at time 0, was arbitrarily set to 1. (D) DNA histograms of G1-synchronised wild-type (left) and gcn2 (right) cells that were untreated (control; shaded histogram) or treated with 5 mM of H2O2 (unshaded) and incubated for the times indicated (in minutes) after H2O2 treatment. (E) Pre-RC assays of G1-synchronised wild-type (left) or gcn2 mutant (right) cells after exposure to 0 (control) or 5 mM of H2O2 and incubated for different periods of time, as indicated.





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