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Fig. 2. Kinetics of stress-induced Calcofluor-white staining patterns. (A) After growth in YES media at 30°C, wild-type cells were subjected to stress, processed as in Fig. 1 and the indicated features scored at the indicated time points. (B) Images of the 60-minute post-sorbitol sample showing septating cells with SICS at their tips (upper panel) or separating cells with septal remnants at one tip and SICS at the other tip (lower panel). (C,D) The indicated strains were processed and scored, as in A, at the indicated time points. Scale bars: 5 µm.