|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 8. SICS, tip growth and cell morphology in sorbitol-treated cells lacking the SRP MAP kinase Sty1. (A) sty1.
cells that were processed, as described in Fig. 1A, at the indicated times are shown. (B,E) Wild-type and sty1. cells grown as in A were fixed and processed for Calcofluor staining to monitor SICS appearance at cell tips (B) and cell morphology (E). (C,D) Wild-type, sty1., atf1. and srk1. cells were coated with fluorescent lectin at t=0 to monitor tip growth at the indicated time points. (D) Representative images of sty1. cells that were coated with fluorescent lectin immediately after sorbitol treatment, returned to sorbitol medium for 300 minutes and then stained with Calcofluor. Calcofluor staining, top; lectin staining, middle; merged images (lectin in green, Calcofluor in red), bottom. (F) Wild-type and cdc2.33 cells were grown at 36°C for 2 hours prior to the addition of sorbitol to a final concentration of 1.2 M, then incubated at 36°C post-stress. The number of cells with morphology defects was determined by fixation and Calcofluor staining to generate the plot shown. (G) Quantification of the types of aberrant phenotype seen at the time points at which the occurrence of cells with defective morphology is highest in sorbitol-treated sty1. cells [210 minutes post-stress (E)] and cdc2.33 cells [120 minute post-stress (F)]. Scale bars: 5 µm.
|
