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Figure 2


Fig. 2. Involvement of activated Src in Cx43-GFP gap junction plaque endocytic internalization. (A-D) Time-dependent effects of HCH on activation of Src and the MAPK pathway (i.e. ERK, p38 and JNK). Sertoli cells were cultured in the presence of 50 µM HCH for increasing times (0, 3.5, 7.5, 30 and 60 minutes). At the indicated times, cells were lysed and levels of activated (i.e. phosphorylated) Src (A), ERK (C), p38 and JNK (D) were assessed by western blotting as described in Materials and Methods (top panels of A, C and D). Total Src and total ERK1/2 levels were also identified (A, C lower panels). Densitometric scanning of Src phosphorylation during exposure to HCH is indicated in B. Similar quantification was carried out for phosphorylation of p42 and p44 (ERK2 and ERK1, respectively) (data not shown). A representative blot of three different experiments is shown for each experiment. * P<0.05 significantly different from time 0.





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