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Figure 3


Fig. 3. Both full-length HDAC6 and the isolated CAT1 and BUZ domains interact with FAT10 in vitro. The diglycine motif of FAT10 is not required for its interaction with HDAC6. (A) GST-pulldown of [35S]-methionine-labeled in vitro transcribed and translated HDAC6 mutants with recombinant FAT10. Bound proteins were analyzed by SDS-PAGE and autoradiography. wt, wild type; CAT1, first catalytic domain; BUZ, ubiquitin-binding zinc finger. (B) Coomassie-staining of the recombinant proteins used in A. (C) GST-pulldown of [35S]-methionine-labeled HDAC6 with recombinant wild-type FAT10 or FAT10 lacking the C-terminal diglycine motif ({Delta}GG). (D) Coomassie-staining of recombinant FAT10 used in C.





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