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Figure 5


Fig. 5. FAT10 localizes to aggresomes under proteasome inhibition. (A) HEK293T cells transiently transfected with HA-FAT10 and FLAG-HDAC6 were treated with 5 µM MG132, or DMSO as a negative control, for 6 hours and immunostained with antibodies for HA (green), FLAG (red) and DAPI (blue; a-h). HEK293T cells transfected with HA-FAT10 were treated with 5 µM MG132 or DMSO for 6 hours and immunostained with anti-HA (green), DAPI (blue) and either anti-{gamma}-tubulin (red; i-p), anti-ubiquitin (red; q-t) or anti-20S proteasome (red; u-x). (B) HEK293T cells treated with TNF-{alpha} and IFN-{gamma} for 16 hours were incubated in the presence of 5 µM MG132 or DMSO for an additional 6 hours and immunostained with antibodies against endogenous FAT10 (green), ubiquitin (red) and DAPI (blue). Scale bars: 5 µm.





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