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Figure 9


Fig. 9. Both of the ubiquitin-like domains of FAT10 interact with HDAC6 and localize to aggresomes under proteasome inhibition. (A) HEK293T cells transfected with FLAG-HDAC6 and the indicated GFP fusions were treated with 5 µM MG132 for 6 hours before lysis, anti-FLAG immunoprecipitation (IP) and analysis by western blot (WB). FAT10-N-GFP, N-terminal domain of FAT10 fused to GFP; FAT10-C-GFP, C-terminal domain of FAT10 fused to GFP. (B) HEK293T cells transfected with either the N-terminal (a-c) or C-terminal (d-f) domain of FAT10 fused to GFP were treated with 5 µM MG132 for 6 hours and immunostained with anti-GFP antibody (green) and DAPI (blue). Scale bars: 5 µm.





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