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Figure 5


Fig. 5. Downregulation of N-cadherin results in enhanced motility of HER2VEKA cells. (A) Wound-healing assays of HER2VEKA-expressing cells treated with siRNA targeting N-cadherin (siNcad). Lines indicate the initial wound edges. Bar, 100 µm. (B) Wound closures of siNcad-treated and -untreated HER2VEKA cells were measured and are given as the percentage of the initial distance. Values are the means ± s.d. from at least three independent experiments. (C) Western blot analysis of HER2VEKA cells treated with siNcad for 24, 48 and 72 hours, compared with untreated HER2VEKA cells. (D) Immunofluorescence staining of HER2VEKA cells treated with siNcad siRNA for 24 and 48 hours, or left untreated. Bar, 20 µm.





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