spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Immunoblot analysis of sumoylation. The genotypes are shown above the lanes; wt, wild type; hmz, homozygous; het, heterozygous. Molecular mass in kilodaltons (kDa) is shown to the left of each panel. (A) Whole-embryo extracts immunoblotted with anti-SUMO1 antibody. There is complete lack of high molecular mass SUMO1 conjugates (upper panel) and free SUMO1 (lower panel) in homozygotes, and reduced levels in heterozygotes. (B) Same blot as in upper panel of A, reanalyzed with anti-RanGAP1 antibody. Sumoylated form of RanGAP1 is present even in homozygotes. (C) Whole-embryo extracts immunoblotted with anti-SUMO3 antibody. Band at the position of sumoylated RanGAP1 (>75 kDa) is more intense in homozygotes. Higher molecular mass SUMO2/3 conjugates are essentially unchanged.





Right arrow Return to article