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Figure 1


Fig. 1. Comparison of microtubule dynamics in the mor1-1 mutant and the wild type. (A) Wild type at 21°C (corresponds to Movie 1 in supplementary material); (B) wild type at 31°C (corresponds with Movie 2 in the supplementary material); (C) mor1-1 at 21°C (corresponds to Movie 3 in supplementary material); (D) mor1-1 at 31°C (corresponds with Movie 4 in supplementary material). Representative time-lapse series of microtubules labelled by expression of the 35S driven GFP-TUB reporter are shown, as collected from epidermal cells on the abaxial surface of first leaves from 11- to 12-day-old seedlings. Microtubule plus ends are indicated with yellow dots and minus ends are labelled with blue triangles. Time between frames was 8 seconds (see supplementary material Movies 1-4) but 16 second intervals are shown here. Images were collected with a spinning disc confocal microscope equipped with a temperature-controlled stage to keep specimens at 21°C or 31°C. Bars, 5 µm.





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