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Fig. 4. EB1-GFP comet tracking of microtubule plus end dynamics in wild type and mor1-1. (A) Representative time-lapse images are displayed so that the relative velocities of EB1 comets are easily compared at 21°C and 31°C for wild type and mor1-1. Images were collected from the abaxial surface of first leaves of 11- to 12-day-old plants. The images of the wild type were obtained from single cells at both temperatures using identical confocal settings and digital processing. Contrast adjustments for the mor1-1 images shown here varied, so the fluorescent intensities cannot be compared. (B) Quantification of ProEB1::EB1-GFP comet velocities in wild type and mor1-1 at 21°C and 31°C. Data were generated from time-lapse images taken every 5 seconds, as shown in A. The velocities were measured from 296 comets from 18 cells from five different plants for wild type at 21°C, 126 comets from 12 cells from three different plants for wild type at 31°C, 157 comets from 10 cells from three different plants for mor1-1 at 21°C and 142 comets from 12 cells from five different plants for mor1-1 at 31°C. Bars indicate the standard deviation.
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