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Figure 5


Fig. 5. Effect of pharmacological or genetic inactivation of class IA PI3K isoforms on DNA synthesis in BAC1.2F5 cells and BMMs. (A) Effect of the p110{delta} inhibitor IC87114 or the pan-PI3K inhibitor LY294002 on DNA synthesis induced in BAC1.2F5 and WT BMM cells by increasing doses of CSF1. (B) Effect of pharmacological inactivation of p110{alpha}, p110β or p110{delta} on DNA synthesis in BMMs and BAC1.2F5 cells induced by CSF1 (30 ng/ml). [3H]Thymidine incorporation of inhibitor-treated cells was expressed relative to that of cells treated with vehicle (DMSO) only (set as 100%). *P<0.05, compared with DMSO-treated cells. (C) Effect of homozygous deletion of p110{alpha} on DNA synthesis in BMMs. The inset shows the expression level of p110{alpha} in Cre– and Cre+ BMMs in a representative experiment. (D) Summary of impact of p110 isoform neutralisation on CSF1-induced DNA synthesis in macrophages.





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