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Figure 7


Fig. 7. (A) Expression levels of class IA PI3K isoforms in BAC1.2F5 cells and BMMs (WT or expressing inactive p110{alpha} or p110{delta}) were determined by immunoblotting of total cell lysates (80 µg per lane). (B) Selective recruitment of p110{delta} to the CSF1R in BMMs but not in BAC1.2F5 cells. WT BMMs or BAC1.2F5 cells were incubated with CSF1 (30 ng/ml) for the indicated times, followed by lysis and immunoprecipitation of p110 isoforms. The co-precipitated CSF1R was detected by western blotting. Equal loading was assessed by reprobing the membrane with antibodies against p85. TCL, total cell lysate (100 µg). For BMMs, a representative experiment out of three is shown. For BAC1.2F5 cells, the results of two independent, representative experiments are shown.





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