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Fig. 2. TNGW1 mRNA containing the TNR exon detected in human testis and different cell lines using RT-PCR. The primer set that flank the TNR region of TNGW1 mRNA (nucleotides 362-1626) amplified 1.2 kb bands from cDNA samples of human testis tissue, HEp-2, HeLa and HepG2 cells. The amplified 1.2 kb products from HEp-2, HeLa and human testis tissue were subsequently purified and their consensuses to reference sequence were confirmed by direct DNA sequencing.