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First published online December 3, 2008

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Old age brings changes for hMSCs

Adult human mesenchymal stem cells (hMSCs) can differentiate into several cell lineages – consequently, they have promise as therapies in regenerative medicine. When grown in culture, however, hMSCs rapidly undergo senescence, which could impact on their use as therapeutic agents. Nuclear structural alteration is a key feature of senescence in many cell types; now, Vered Raz and colleagues (p. 4018) use newly developed quantitative image-processing tools to analyse how the nuclear architecture of hMSCs changes during senescence. The authors show that, early in senescence, the nuclear lamina of hMSCs becomes deformed and gives rise to intranuclear structures; in addition, centromeres and telomeres of chromosomes relocalise to a peripheral nuclear position that spatially overlaps with these structures. The authors observe that, during senescence, telomeres form large aggregates that colocalise with the DNA-damage marker -H2AX but not with the telomerase component TERT. Notably, the binding of telomere aggregates to lamina structures is enhanced when the lamina is distorted through the expression of mutant lamins. Thus, the nuclear lamina of senescent hMSCs regulates the spatial positioning of heterochromatic chromosome regions. These data shed light on how nuclear architecture changes during hMSC senescence.

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Related articles in JCS:

The nuclear lamina promotes telomere aggregation and centromere peripheral localization during senescence of human mesenchymal stem cells

Vered Raz, Bart J. Vermolen, Yuval Garini, Jos J. M. Onderwater, Mieke A. Mommaas-Kienhuis, Abraham J. Koster, Ian T. Young, Hans Tanke, and Roeland W. Dirks
JCS 2008 121: 4018-4028. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?