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Figure 7


Fig. 7. PLD1 regulates myogenesis through IGF2 production. C2C12 cells were infected with lentiviruses expressing various shRNAs, selected with puromycin, and then induced to differentiate for 3 days with or without exogenous IGF2 (300 ng/ml), followed by (A) western analysis of the cell lysates, or (B) immunostaining of MHC (green) and DAPI (red). (C) The average results of fusion index calculated from three independent experiments in B. Error bars represent s.d.





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