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Figure 1


Fig. 1. Apoptosis is initiated in human glioma cells in response to staurosporine (STS) and TRAIL treatment. (A) Caspase activity was measured in D54-MG cells treated with normal bath, 1 µM STS or 50 ng/ml TRAIL by a luminescence assay. Each time point was normalized to the luminescence of cells after 0 hours of treatment with normal bath. Data are from three experiments. Error bars represent s.e.m. and n=3 samples per experiment. (B) Western blots of cell lysates from cells treated for 1, 3 or 7 hours were probed with antibodies against the cleaved, activated forms of caspases 3, 8 and 9. (C) Representative images demonstrate DNA fragmentation (fluorescein-labeled nuclei) after treatment with normal bath, STS or TRAIL for 7 hours. Merged images show both fragmented nuclei (green) and total nuclei present by DAPI staining (blue). Bar, 100 µm.





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