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Figure 1


Fig. 1. Cytokinesis in toroidal C. elegans embryos. (A) Complete perforation interposes a cylinder of cell cortex between the mitotic apparatus and what would have been the equatorial cortex; no latitudinal connection exists between the cortex surrounding the mitotic apparatus and the cortex on the opposite side of the probe. (B) Frames from a DIC film of perforation and subsequent cytokinesis (supplementary material Movie 1). Frame 1 shows the dechorionated zygote at metaphase, still in its vitelline envelope. Anterior is to the left in all figures. Frame 2 is immediately following perforation with a probe ~5 µm in diameter. Arrowheads indicate spindle poles; ss, spindle side, fs, far side from the perforation. A furrow forms on both the far side and spindle side (frame 3); in this case, both furrows ingress deeply (frame 4), but the far-side furrow regresses (frame 5) shortly after nuclei reappear. The spindle-side furrow completes (frame 6), yielding a U-shaped, binucleate cell. Bar, 10 µm.





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