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Figure 2


Fig. 2. Results from perforation of wild-type zygotes (P0) and the anterior cell (AB) of the two-cell embryo. Numbers shown include all cases, whether filmed with transmitted light or using NMY-2::GFP, in which perforation unambiguously preceded anaphase onset. (A) Most perforated P0 cells exhibit two overt furrows, but in the vast majority only the spindle-side furrow is stable. If the probe fails to intercept the space between the spindle midzone and the equator, then typically a furrow forms around the equator, seemingly ignoring the probe. In some cases the cortex surrounding the probe participates, and may yield a U-shaped binucleate cell, or cleavage may eliminate the probe from both cells. (B) In perforated AB, overt furrowing is less frequent on the far side of the probe (although the far side always exhibits myosin recruitment; see Fig. 3). As in P0, almost all AB in which the probe lies directly between the mitotic apparatus and the far-side cortex, successfully cleave only on the spindle side, yielding a binucleate U-shaped cell.





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