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Figure 7


Fig. 7. Fab fragments against the C-terminal domain of gp210 block mitotic phosphorylation. Membranes were pre-incubated with no, control or inhibitory Fab fragments. Interphase or mitotic extracts and radioactive [{gamma}-32P]ATP were added and incubated for 20 minutes. The reaction was stopped by solubilization of the membranes, gp210 was immunoprecipitated and the samples analyzed by autoradiography (upper panel) or western blotting (lower panel).





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