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Figure 8


Fig. 8. RNAi depletion of cyclin B leads to formation of twinned nuclei and blocks lamin depolymerization. (A) Confocal still images from time-lapse recordings of the first zygotic divisions showing cyb-1(RNAi) two-cell-stage (top) or four-cell-stage (bottom) embryos expressing GFP–β-tubulin. The twinned nuclei (arrows) induced upon RNAi depletion of cyclin B exclude soluble GFP-β–tubulin. (B) Confocal still images from time-lapse recordings of the second and third zygotic divisions showing cyb-1(RNAi) embryos expressing YFP-lamin. Localized YFP-lamin staining (arrowheads) remains during mitosis upon RNAi depletion of cyclin B. Bars, 10 µm.





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