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Fig. 2. Genetic interactions between SYN8, ENT3 and ENT5. (A) The absence of Ent3p and Ent5p led to a synthetic growth defect in syn8
cells at 30°C and 37°C. (B) Absence of Ent3p and Ent5p resulted in synthetic defects of CPY transport to the vacuole in syn8
cells. CPY was immunoprecipitated from cellular extracts (I) and the medium (E) after pulse-chase labeling at 30°C. p1CPY, endoplasmic reticulum pro1CPY; p2CPY, Golgi pro2CPY; mCPY, vacuolar mature CPY. (C) Quantification of CPY secretion. Data for WT, ent3
and ent5
cells were from a previous experiment. n=2, Bars indicate range of values. Strains used in this figure were BY4742 (WT) and derivates: syn8
, JZY1, JZY3, JZY2, ent3
, ent5
, BKY13