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Figure 6


Fig. 6. Pep12p and Vti1p were redistributed to denser fractions in ent3{Delta} ent5{Delta} and syn8{Delta} ent3{Delta} ent5{Delta} cells on a sucrose density gradient. Cleared yeast homogenates from wild-type (BY4742), syn8{Delta}, ent3{Delta} ent5{Delta} (BKY13) and syn8{Delta} ent3{Delta} ent5{Delta} (JZY2) cells were fractionated on a 19% to 42% sucrose density gradient and analyzed by immunoblotting for Pep12p (A) and Vti1p (B). The vacuolar 60 kDa ATPase (C) was present in the vacuolar fractions (3-5) and ER was found in fractions 9-11 as indicated by the marker Use1p (D).





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