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Figure 8


Fig. 8. A defective FSD motif in Pep12p had similar effects on Pep12p stability as absence of Ent3p. Cells were labeled with [35S]methionine for 25 minutes and chased for 10 minutes, 3 hours or 5 hours. Pep12p was immunoprecipitated from the cellular extracts and the percentage of Pep12p remaining after 3 and 5 hours was calculated. Strains used: WT, SEY6210; ent3{Delta}, SCY2; F20L, SCY27 (pep12{Delta}::URA3) pJZ11 (CEN PEP12-F20L).





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