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Fig. 8. 185/333 proteins are a complex mixture of sizes. Coelomocytes were collected from two sea urchins (animals 7 and 20) and analyzed by western blot with the anti-185/333 antisera. (A) Individual sea urchins show differences in the range of 185/333 sizes. Standards are shown to the left. (B) The amount of 185/333 proteins in coelomic fluid increased after injection with LPS. Coelomocytes were collected from animal 7 before injection with LPS (lane 1) and after injection at 1 hour (lane 2), 6 hours (lane 3) and at 24 hours (lane 4).