spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Silencing of NBS1 increases formation of rAAV foci. (A) Western blotting showing the levels of NBS1 in HeLa cells after treatment with siRNAs against NBS1 (siNbs1) or luciferase (siLuc) at 60 hours after siRNA transfection (corresponding to 24 hours p.i.). The western blotting against tubulin was used as a loading control. (B) Representative images of cells treated with anti-NBS1 or anti-Luc siRNAs followed by transduction with AAV-LacO.14. The images were taken at 24 hours p.i. without HU treatment. (C) Percentage of HeLa cells with detectable AAVLacO.14 foci after silencing of NBS1. Cells were evaluated for the presence of rAAV foci 16 and 24 hours after transduction, in the presence or absence of HU treatment, as indicated. (D) Number of AAVLacO.14 foci per cell after silencing of NBS1. Conditions were the same as in C.





Right arrow Return to article