spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. EPHA2 regulates adherens junction stability via modulation of RhoA activity. (A) Activated Rho and Rac GTPases in MCF-10A, MCF-10A-EPHA2, or MCF-10A-{Delta}C cells in response to ephrin-A1 stimulation were measured by GST-Rhotekin binding domain and GST-Pak binding domain pull-down assays, respectively. Total levels of Rho and Rac proteins were assay by western blot analysis. *P<0.05. (B-E) MCF-10A, MCF-10A-{Delta}C, MCF-10A-EPHA2 or MCF-10A-EPHA2 treated with ROCK kinase inhibitor, Y27632, were subjected to Ca2+ depletion for 8 hours, followed by detection of E-cadherin. (F-I) MCF-10A and MCF-10A-{Delta}C cells expressing a constitutively active Rho (Q63L) or control β-galactosidase (LacZ) were assayed for adherens junction stability by Ca2+-depletion assay. (J) The expression and activity of Q63L in control and MCF-10A-{Delta}C cells were confirmed by Rhotekin pull-down assays and western blot analysis.





Right arrow Return to article