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Figure 5


Fig. 5. EPHA2 regulates adherens junction stability through p190 RhoGAP. (A) p190 RhoGAP was immunoprecipitated from MCF-10A, MCF-10A-{Delta}C, or MCF-10A-EPHA2 cell lysates and blotted for tyrosine phosphorylation. Tyrosine phosphorylation of p190 was decreased in MCF-10A-EPHA2 cells. (B-I) MCF-10A, MCF-10A-EPHA2 and MCF-10A-{Delta}C cells expressing p190 or the dominant negative p190 mutant 30-1 were assayed for adherens junction stability by Ca2+-depletion assay. Expression of wild-type p190 rescued the adherens junctions in MCF-10A-EPHA2 cells (I), whereas expression of the 30-1 mutant destabilized cell-cell adhesion in parental MCF-10A cells and MCF-10A-{Delta}C cells (D,G).





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