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Fig. 8. Level of EPHA2 overexpression determines rate of adherens junction disassembly. (A) Expression and tyrosine phosphorylation of EPHA2 receptor in various MCF-10A cells and two breast cancer cell lines, BT-549 and MDA-MB-231, were determined by immunoprecipitation and western blot analysis. (B) MCF-10A, MCF-10A-LZRS-EPHA2, MCF-10A-LacZ, or MCF-10A-Ad/EPHA2 cells were immunostained for E-cadherin localization in the presence of normal or low Ca2+ medium for 2 hours.