spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Depletion of cortactin by RNA interference. (A) Lysates of A375MM cells 72 hours after mock or cortactin-specific siRNA transfection were subjected to SDS-PAGE, transferred to nitrocellulose and probed with anti-cortactin and anti-GAPDH as a loading control. (B) The relative amount of cortactin in mock-transfected and knockdown cells was measured. A 95% reduction in the amount of cortactin was observed. (C) Immunofluorescence labeling with anti-cortactin and phalloidin of cortactin siRNA-treated A375MM cells. Depletion of cortactin blocks the formation of invadopodia and hence, matrix degradation. (D) Transfection of cortactinWT 48 hours after knockdown. Normal invadopodia form and degrade the extracellular matrix. Scale bars: 10 µm.





Right arrow Return to article