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Fig. 1. The localization of IRSp53 in macrophages. BMM (top panel) or RAW/LR5 cells (bottom panel) were treated, or not, with 20 ng/ml CSF-1 for 5 minutes and then fixed and stained for F-actin using Alexa-568-phalloidin and for IRSp53 using a polyclonal goat antibody followed by Alexa-488-coupled anti-goat IgG. Bar, 10 µm.
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