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Fig. 4. Confocal images of IQGAP1 co-localization with EXO70. Upper panels: fixed HeLa cells were double-stained with antibodies for IQGAP1 (Texas red) and EXO70 (Alexa-Fluor-488, green) and examined with a confocal microscope. Lower panels: Left, confocal slice (
0.2 µm) of the perinuclear area. Middle, thin confocal slice (
0.18 µm). Right, high-resolution image of the merged section in the upper panel for the cytoplasmic tubular structures (arrow) of IQGAP1-EXO70. No post-image-acquisition processing was carried out.