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Figure 5


Fig. 5. Distal tip localization of Ift172p-HA in the IFT122A knockout cells. (A) Strategy to generate tagged strains with IFT172-HA knocked-in to the endogenous locus. (B) Growth curves of the CU428 wild-type cells and IFT knockout cells. (C-H) Immunofluorescence staining of the cells with wild-type IFT122A (C,D) or with IFT122A deleted (E-H). Cells were stained with anti-HA monoclonal antibody (red) and anti-tubulin antiserum (green), and D, F and H are merged images of Ift172p-HA and tubulin staining. Ift172p-HA became enriched at the distal region of some cilia (arrow). Scale bars, 10 µm.





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