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Figure 6


Fig. 6. Localization of Ift172p-HA during cilia regeneration in the IFT122A knockout cells. IFT122A knockout cells with HA-tagged IFT172 were deciliated by pH shock and allowed to regenerate cilia. Cells collected after 20 minutes (A-C), 40 minutes (D-F) and 60 minutes (G-I) in the regeneration buffer were fixed and stained with anti-tubulin (green) and anti-HA antibody (red). The Ift172p-HA first appeared in nascent cilia and gradually became enriched at the tips of some cilia. Arrowhead, nascent cilia at 20 minutes. Arrow, the Ift172p-HA in the nascent cilia (C) and accumulation at the tips of cilia (F and I). Scale bar, 10 µm.





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